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MODERATE OC TEMPLATE. Contributor. π–€β€”π“π€π„π‘πˆπ! 55. 17. 55. 17 ⍟ small quote small quote small quote. We determined overall DNA synthesis ability as well as the efficiency and fidelity of bypass of thymine-thymine CPD (T-T CPD) and 8-oxoG containing DNA templates. Our results show that several amino acids are critical for normal polymerase function, with changes in overall activity and fidelity being observed.

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Optimization of PCR Conditions for a Template with Moderate GC Content In order to establish a new PCR assay, choosing an adequate annealing temperature and an appropriate polymerase is necessary. The calculated annealing temperature for the TGFΞ²3 PCR was 51 Β°C.
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